Tag: Selecting oligonucleotide

There are a few vital considerations when choosing oligonucleotides for your study to ensure the function of the sequence as planned in your tests. The primary factors that will help you select the best oligonucleotides for your project are listed below. Sequence Design The experiment's efficiency and specificity depend heavily on the oligonucleotide sequence. Being complementary to the target DNA or RNA region is the first step in creating an appropriate oligonucleotide. For the PCR method, sequencing, or gene silencing necessitates choosing a sequence that will hybridize firmly to the template and ensure better binding. Unwanted secondary structures, repetitions, and complimentary sequences that could result in non-specific binding should be removed from the sequence. Online oligonuc...